中美垃圾场渗滤液中的金属成分变化的调研

主要对2000～2004年美国佛罗里达州中部的5个垃圾场中垃圾渗滤液的金属离子成分进行统计分析,从中发现锑(Sb)、砷(As)、铬(Cr)、铁(Fe)、镍(Ni)、钠(Na)、钒(V)、硒(Se)等8种金属在渗滤液中的质量浓度超过EPA地表及饮用水标准,其中比较严重的是As,Fe和Na,每年都超EPA标准.推测该地区地表或地下水体被As,Fe,Na污染的可能性很大.对其他还未列入监控标准的金属也做了来源及危害分析.同时与国内某垃圾场渗滤液中的金属成份进行了比较,结果显示该垃圾场渗滤液只有汞(Hg)超国家V类水体标准,但还在美国EPA容许范围内.     

Soil microbial response to herbicides applied to glyphosate-resistant canola

Adoption of glyphosate-resistant canola (Brassica napus L.) has increased glyphosate applications to this crop, and concerns have been raised about unintended consequences of these multiple applications. A field trial was conducted to evaluate the effects of pre-seed and in-crop glyphosate and alternative herbicides on soil microbial community functional structure, diversity and biomass. Pre-seed treatments were 2,4-D, glyphosate and 2,4-D + glyphosate, and in-crop treatments were glyphosate applied once, glyphosate applied twice, ethalfluralin, ethalfluralin + sethoxydim + ethametsulfuron + clopyralid, and sethoxydim + ethametsulfuron. Rhizosphere and bulk soil was collected at flowering stage of canola and analyzed for bacterial community-level substrate utilization patterns and microbial biomass C (MBC). Where differences were significant, pre-seed application of both 2,4-D and glyphosate altered the functional structure and reduced the functional diversity of soil bacteria, but increased MBC. These effects were not necessarily concurrent. The reduction in functional diversity was due to reduction in evenness, which means that the soil where both pre-seed herbicides had been applied was dominated by only few functional groups. In 1 year, two in-crop applications of glyphosate also reduced the functional diversity of soil bacteria when applied after pre-seed 2,4-D, as did in-crop sethoxydim + ethametsulfuron following pre-seed glyphosate. Even though significant differences between herbicides were fewer than non-significant differences, i.e., there were no changes in soil microbial community structure, diversity or biomass in response to glyphosate or alternative herbicides applied to glyphosate-resistant canola in most cases, the observed changes in soil microbial communities could affect soil food webs and biological processes.     

European scale application of atmospheric reactive nitrogen measurements in a low-cost approach to infer dry deposition fluxes

A European scale network was established in 2006 as part of the NitroEurope Integrated Project to infer reactive nitrogen (Nr) dry deposition fluxes, based on low-cost sampling of gaseous and aerosol species and inferential modelling. The network provides monthly measurements of NH₃, NH₄⁺, HNO₃ and NO₃⁻, as well as SO₂, SO₄²⁻, HCl, Cl⁻ and base cations at 58 sites. Measurements are made with an established low-cost denuder methodology (DELTA) as a basis to: (1) examine temporal trends and spatial patterns across Europe, (2) improve and calibrate inferential modelling techniques to estimate exchange of Nr species, (3) provide best estimates of atmospheric dry N deposition, and (4) permit an analysis of net GHG exchange in relation to atmospheric and agricultural N inputs at the European scale. Responsibility for measurements is shared among seven European laboratories. An inter-comparison of the DELTA implementation by 6 laboratories at 4 test sites (Montelibretti, Italy; Braunschweig, Germany; Paterna, Spain and Auchencorth, UK) from July to October 2006 provided training for the laboratories and showed that good agreement was achieved in different climatic conditions (87% of laboratory site-means within 20% of the inter-laboratory median). Results obtained from the first year of measurements show substantial spatial variability in atmospheric Nr concentrations, illustrating the major local (NH₃) and regional (HNO₃, NO₃⁻, and NH₄⁺) differences in Nr concentrations. These results provide the basis to develop future estimates of site-based Nr dry deposition fluxes across Europe, and highlight the role of NH₃, largely of agricultural origin, which was the largest single constituent and will dominate dry Nr fluxes at most sites.     

Changes in functional structure of soil bacterial communities due to fungicide and insecticide applications in canola

The fungicide vinclozolin and insecticide λ-cyhalothrin are widely used to control canola (Brassica spp.) diseases and insect pests, respectively, in Canada. We investigated non-target effects of these pesticides, applied at recommended rates, on soil microbial biomass, functional bacterial diversity and functional community structure of soil bacteria (by evaluating patterns of C substrate utilization) in canola rhizosphere and bulk soil at three locations in Alberta from 2002 to 2004. Experimental treatments were (a) untreated control, (b) vinclozolin fungicide foliar application, (c) λ-cyhalothrin insecticide foliar application, and (d) vinclozolin and λ-cyhalothrin applications. No significant pesticide effects on soil microbial biomass or functional bacterial diversity were observed, but the functional structures of soil bacteria were altered. In 1 of 12 cases, the control treatment had a different soil bacterial community structure from the 3 pesticide treatments. The fungicide treatment had different bacterial community structures from the control or insecticide treatments in 3 of 12 cases, the insecticide treatment had different community structures from the control or fungicide treatments in 4 of 12 cases, and the combined fungicide and insecticide treatment had different community structures from the other treatments in 3 of 12 cases. Therefore, evaluating soil bacterial functional structures revealed pesticide effects that were not detected when bacterial diversity or microbial biomass were measured in canola rhizosphere or bulk soil.     

以膨润土为辅助添加剂固化/稳定化污泥的试验研究

针对传统以水泥固化污泥,带来的水泥用量大与固化体的浸出液pH过高等问题,提出了以膨润土为添加剂辅助水泥固化/稳定化污泥的思路.通过开展无侧限抗压强度试验、毒性浸出试验,测量掺入膨润土后污泥固化体的强度、重金属浸出率、浸出液COD及pH值,研究该固化/稳定化方法的效果.结果表明,膨润土的掺入极大地提高了固化体的抗压强度,将掺入量为0.4(相对污泥的质量比)的水泥一半用膨润土替代时,固化体的强度提高了6左右.体积安定性也能够满足要求.随膨润土掺入量增加,固化体中锌、铅的浸出率与浸出液的pH值呈现不断减小的趋势,锌与铅的浸出率分别由6.9%下降至0.25%,9.6%下降至5%,pH值由12.3下降至12.1.在强碱条件下及烘干或风干条件下,铜会随着有机物的分解而析出,从而增加铜的浸出率,而膨润土的加入能弥补水泥造成的强碱环境及风干或烘干过程对固化污泥中铜的稳定产生的不利影响.     

Effect of rhizosphere on soil microbial community and in-situ pyrene biodegradation

To access the influence of a vegetation on soil microorganisms toward organic pollutant biogegration, this study examined the rhizospheric effects of four plant species (sudan grass, white clover, alfalfa, and fescue) on the soil microbial community and in-situ pyrene (PYR) biodegradation. The results indicated that the spiked PYR levels in soils decreased substantially compared to the control soil without planting. With equal planted densities, the efficiencies of PYR degradation in rhizosphere with sudan grass, white clover, alfalfa and fescue were 34.0%, 28.4%, 27.7%, and 9.9%, respectively. However, on the basis of equal root biomass the efficiencies were in order of white clover >> alfalfa > sudan > fescue. The increased PYR biodegradation was attributed to the enhanced bacterial population and activity induced by plant roots in the rhizosphere. Soil microbial species and biomasses were elucidated in terms of microbial phospholipid ester-linked fatty acid (PLFA) biomarkers. The principal component analysis (PCA) revealed significant changes in PLFA pattern in planted and non-planted soils spiked with PYR. Total PLFAs in planted soils were all higher than those in non-planted soils. PLFA assemblages indicated that bacteria were the primary PYR degrading microorganisms, and that Gram-positive bacteria exhibited higher tolerance to PYR than Gram-negative bacteria did.     

Runoff and sediment losses from 27 upland catchments in Southeast Asia: Impact of rapid land use changes and conservation practices

Rapid changes in upland farming systems in Southeast Asia generated predominantly by increased population pressure and ‘market forces’ have resulted in widespread land degradation that has been well documented at the plot scale. Yet, the links between agricultural activities in the uplands and downstream off-site effects remain largely unknown because of the difficulties in transferring results from plots to a larger scale. Many authors have thus pointed out the need for long-term catchment studies. The objective of this paper is to summarize the results obtained by the Management of Soil Erosion Consortium (MSEC) over the last 5 years from 27 catchments in five countries (Indonesia, Laos, Philippines, Thailand, and Vietnam). The purpose of the study was to assess the impacts of cultivation practices on annual runoff and erosion rates. Initial surveys in each catchment included topography, soils and land use. Monitoring included climatic, hydrologic and erosion (total sediment yield including bed load and suspended sediment load) data, land use and crop yields, and farmers’ income. In addition, new land management options were introduced through consultations with farmers and evaluated in terms of runoff and erosion. These included tree plantations, fruit trees, improved fallow with legumes, maize intercropped with legumes, planted fodder, native grass strips and agro-ecological practices (direct sowing and mulch-based conservation agriculture). Regressions analyses showed that runoff during the rainy season, and normalized runoff flow coefficient based on erosive rainfall during the rainy season (rainfall with intensity exceeding 25 mm h⁻¹) increase with the percentage of the catchment covered by maize. Both variables decrease with increasing soil depth, standard deviation of catchment slope (that reflects terrain roughness), and the percentages of the catchment covered by fallow (regular and improved), tree plantations and planted fodder. The best predictors of sediment yield were the surface percentages of maize, Job's tears, cassava and footpaths. The main conclusions generated from this study were: (i) soil erosion is predominantly influenced by land use rather than environmental characteristics not only at the plot scale but also at the catchment scale; (ii) slash-and-burn shifting cultivation with sufficiently long rotations (1 year of cultivation, 8 years of fallow) is too often unjustly blamed for degradation; (iii) in its place, continuous cropping of maize and cassava promotes high rates of soil erosion at the catchment scale; (iv) conservation technologies are efficient in reducing runoff and total sediment yield at the catchment scale; (v) the adoption of improved soil management technologies by upland farmers is not a function of the degree of intensification of their farming system and/or of their incomes. The results suggest that if expansion of maize and cassava into already degraded upland systems were to occur due to increased demand for biofuels, there is a risk of higher runoff and sediment generation. A failure to adopt appropriate land use management strategies will result in further rapid resource degradation with negative impacts to downstream communities.     

Seasonal ecophysiology of an endangered coastal species,the yellow-horned poppy (Glaucium flavum Crantz)

Glaucium flavum Crantz. is a short-lived perennial herb distributed in coastal zones from the Black Sea to southern, western and north-western Europe. Despite its diminishing area of distribution and potential pharmacological value, little is known about the ecophysiological features of this coastal species. We investigated the photosynthetic performance of G. flavum by measuring gas exchange, chlorophyll fluorescence, photosynthetic pigment concentration and leaf water content over the space of a year in a coastal habitat of SW Spain. We also measured the variation in total concentrations of nitrogen, phosphorus, sulphur, potassium, calcium and magnesium, in the leaves and soil, throughout the study period. G. flavum showed a high resistance to summer drought conditions which appeared to be due to the high degree of stomatal control. The potential photochemical efficiency of photosystem II showed minimum values during the winter, indicating that low temperatures can produce negative effects within the photosynthetic apparatus. However, the marked decline in net photosynthesis during the winter seems to be mainly related to a loss of metabolic activity. Although leaf nutrient concentrations were, in general, within the normal ranges, phosphorus availability seems to be limited by the high calcium concentrations detected in the soil of the study site. Our study points out the efficiency of the different physiological adaptations of this rare and endangered coastal species in coping with the strong seasonal variability of the Mediterranean climate.     

Coagulant recovery from water treatment plant sludge and reuse in post-treatment of UASB reactor effluent treating municipal wastewater

In the present study, feasibility of recovering the coagulant from water treatment plant sludge with sulphuric acid and reusing it in post-treatment of upflow anaerobic sludge blanket (UASB) reactor effluent treating municipal wastewater were studied. The optimum conditions for coagulant recovery from water treatment plant sludge were investigated using response surface methodology (RSM). Sludge obtained from plants that use polyaluminium chloride (PACl) and alum coagulant was utilised for the study. Effect of three variables, pH, solid content and mixing time was studied using a Box–Behnken statistical experimental design. RSM model was developed based on the experimental aluminium recovery, and the response plots were developed. Results of the study showed significant effects of all the three variables and their interactions in the recovery process. The optimum aluminium recovery of 73.26 and 62.73 % from PACl sludge and alum sludge, respectively, was obtained at pH of 2.0, solid content of 0.5 % and mixing time of 30 min. The recovered coagulant solution had elevated concentrations of certain metals and chemical oxygen demand (COD) which raised concern about its reuse potential in water treatment. Hence, the coagulant recovered from PACl sludge was reused as coagulant for post-treatment of UASB reactor effluent treating municipal wastewater. The recovered coagulant gave 71 % COD, 80 % turbidity, 89 % phosphate, 77 % suspended solids and 99.5 % total coliform removal at 25 mg Al/L. Fresh PACl also gave similar performance but at higher dose of 40 mg Al/L. The results suggest that coagulant can be recovered from water treatment plant sludge and can be used to treat UASB reactor effluent treating municipal wastewater which can reduce the consumption of fresh coagulant in wastewater treatment.     

Survival of Escherichia coli in stormwater biofilters

Biofilters are widely adopted in Australia for stormwater treatment, but the reported removal of common faecal indicators (such as Escherichia coli (E. coli)) varies from net removal to net leaching. Currently, the underlying mechanisms that govern the faecal microbial removal in the biofilters are poorly understood. Therefore, it is important to study retention and subsequent survival of faecal microorganisms in the biofilters under different biofilter designs and operational characteristics. The current study investigates how E. coli survival is influenced by temperature, moisture content, sunlight exposure and presence of other microorganisms in filter media and top surface sediment. Soil samples were taken from two different biofilters to investigate E. coli survival under controlled laboratory conditions. Results revealed that the presence of other microorganisms and temperature are vital stressors which govern the survival of E. coli captured either in the top surface sediment or filter media, while sunlight exposure and moisture content are important for the survival of E. coli captured in the top surface sediment compared to that of the filter media. Moreover, increased survival was found in the filter media compared to the top sediment, and sand filter media was found be more hostile than loamy sand filter media towards E. coli survival. Results also suggest that the contribution from the tested environmental stressors on E. coli survival in biofilters will be greatly affected by the seasonality and may vary from one site to another.